| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta
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| Autores: |
Henneke, Ghislaine
Raffin, Jean-paul
Ferrari, Elena
Jonsson, Zophonías
Dietrich, Jacques
Hubscher, Ulrich
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| Data: |
2000-09
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| Ano: |
2000
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| Palavras-chave: |
DNA polymerase delta
Conservation
Thermococcus fumicolans
Archaea
Hyperthermophile
PCNA
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| Resumo: |
We have cloned the gene encoding proliferating cell nuclear antigen (PCNA) from the hyperthermophilic euryarchaeote Thermococcus fumicolans (Tfu). Tfu PCNA contains 250 amino acids with a calculated M-r of 28,000 and is 26% identical to human PCNA. Next, Tfu PCNA was overexpressed in Escherichia coli and it showed an apparent molecular mass of 33.5 kDa. The purified Tfu PCNA was tested first with recombinant Tfu DNA polymerase I (Tfu pol) and second with calf thymus DNA polymerase delta (pol delta). When tested with the homologous Tfu pol on bacteriophage lambda DNA, large amounts of Tfu PCNA were required to obtain two- to threefold stimulation. Surprisingly, however, Tfu PCNA was much more efficient than human PCNA in stimulating calf thymus pol delta. Our data suggest that PCNA has been functionally conserved not only within eukaryotes but also from hyperthermophilic euryarchaeotes to mammals.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2000/publication-659.pdf
DOI:10.1006/bbrc.2000.3481
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/659/
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| Formato: |
application/pdf
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| Fonte: |
Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2000-09 , Vol. 276 , N. 2 , P. 600-606
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| Direitos: |
2000 Academic Press. All rights reserved
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